WebMar 10, 2024 · Highly purified bST (reagent AFP-11182B/AFP-9884C) was used for standards (useful concentration range: 0–100 ng/mL) and for iodination (based on the iodogen method of Salacinski et al., 1981 ). Monkey anti-bovine bST serum (AFP-B55Bb) was used as the primary antibody (1:500,000 final tube dilution). WebJan 21, 2024 · RT-LAMP is commonly performed with two separate enzymes such as Bst DNA polymerase and AMV reverse transcriptase, ... but the protocols are freely published and reagents are readily available from multiple suppliers, which fosters a community of innovation [Citation 12].
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WebJun 22, 2024 · Background This study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR). All the assays demonstrated in this study were developed based on Serine-rich Entamoeba histolytica … WebMay 11, 2011 · Effects of macromolecular crowding reagents. The effects are shown as the difference in detection time (calculated by: 2 × (Tt reaction without crowding agent - Tt … WebAug 27, 2024 · Bone marrow stromal cell antigen 2 (BST-2), also known as CD317 or tetherin, has been identified as a host restriction factor that suppresses the release of enveloped viruses from host cells by physically tethering viral particles to the cell surface; however, this host defense can be subverted by multiple viruses. For example, human … hayfever injection watford high street