WebB Polyubiquitinated proteins associated with UBQLN4 are sensitive to the translation inhibitor cycloheximide (CHX). HeLa cells expressing Flag-tagged UBQLN4 were treated with 5 lM MG-132 as well as CHX (either 10 or 25 lg/ml) for 4 h as indicated and then subjected to immunoprecipitation with an anti-Flag antibody as in (A). WebFIGURE 1 Development of dual-reporter cell lines and a CHX chase assay to monitor UPS function. A, HeLa cells stably expressing Ub G76V-GFP and ODD-Luc were treated with the indicated amounts of MG132 for 2 h. …
CHX File: How to open CHX file (and what it is)
WebCycloheximide solution Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture Synonym (s): CHX Empirical Formula (Hill Notation): C15H23NO4 CAS Number: 66-81-9 Molecular Weight: 281.35 MDL number: MFCD00082346 PubChem Substance ID: 24892732 NACRES: NA.77 Pricing and availability is not currently … WebWe show that HeLa cells overexpressing ERK‐1 displayed resistance to TNF and CHX. HeLa cells overexpressing a kinase‐deficient form of ERK‐1 (K71R) were more sensitive to TNF and CHX. In the ERK‐1 cells, Bad was phosphorylated during TNF + CHX treatment. In the HeLa wt cells and in the K71R clones TNF and CHX decreased Bad phosphorylation. phoenix fund investments difc limited
ERK‐1 MAP kinase prevents TNF‐induced apoptosis through bad ...
WebFigure Legend Snippet: (A) Hela cells were pretreated with CHX (10 µM) for 0.5 h before addition of TPL (125 nM) and treated for another 3 h. Whole cell lysates were processed for Western blot analysis using antibodies as indicated. (B) Hela cells were pretreated with MG132 (20 µM) for 1 h and then treated with TPL at indicated concentrations for another … WebDec 1, 2009 · We show that HeLa cells overexpressing ERK-1 displayed resistance to TNF and CHX. HeLa cells overexpressing a kinase-deficient form of ERK-1 (K71R) were more sensitive to TNF and CHX. In the ERK-1 cells, Bad was phosphorylated during TNF + CHX treatment. In the HeLa wt cells and in the K71R clones TNF and CHX decreased Bad … WebNov 14, 2014 · Note the marked decrease in total cellular ABCG1-GFP with time; (B) Control and ABCG1-GFP stably expressing HeLa cells were labeled with 3 H-cholesterol and efflux to 50 µg/mL HDL in the absence or presence of CHX was assessed as described in “Experimental Section”. Note that CHX had little if any effect on ABCG1-mediated cellular ... phoenix frenchtown mi