Phospho-histone h3-s10
WebAnti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq. Research Category Epigenetics & Nuclear Function WebProduct Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Histone H3 (Ser10) Antibody #9701.
Phospho-histone h3-s10
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WebWestern blot - Phospho-Histone H3-S10 Rabbit pAb (AP0840) Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10 Rabbit pAb (AP0840) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ... WebHistone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified ...
WebPhosphorylation of histone H3, referred to as the nucleosomal response, is localized to a small fraction of highly acetylated H3s and occurs primarily in response to mitogenic and … WebOur in silico data highlighted the potential interaction of MKP-1 with site-specific phospho-acetylated histone H3 at S10, K9 and K14 [23]. Earlier studies have demonstrated that MKP1 interacts ...
WebPhospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. WebDetection of Phospho Histone H3 (pS10). Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 x 10^6 cells were stained with 0.4 µg anti-phospho Histone H3 (S10) (Product # 301-844A) in a 150 µL reaction.
WebThis antibody is specific for Histone H3 phosphorylated at residue Ser 28 and does not recognise the unmodified residue or another phosphorylated residue (Ser 10) on the same histone. Tested applications Suitable for: WB, PepArr, ICC/IF more details Species reactivity Reacts with: Human, Recombinant fragment
WebThe antiviral activity of these compounds correlated with an apparent decrease in histone H3 phosphorylation at serine 10 (H3S10ph) during viral infection, suggesting that the phosphorylation ... shipworks/pinWebPHH3 showed 0 to 16 mitoses/10 HPFs (mean, 3.8) in the STUMP group and 0 to 61 mitoses/10 HPFs (mean, 26) in the LMS group. In 7 cases, the MI difference (MID) between H&E-stained and PHH3-stained slides varied from 0 to 3 mitoses (mean, 1.6). In only one LMS, the PHH3 MI was lower than on H&E-stained slides (MID 7). shipworks reportingWebPhospho-Histone H3 (Ser10) (6G3) Mouse mAb detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not cross-react with other … quickly passingWebEnter the email address you signed up with and we'll email you a reset link. shipworks printer setupWebFeb 1, 2005 · The dynamic distribution of phosphorylated Histone H3 on Ser10 (phospho-H3) in cells was investigated to determine its function during mitosis. Human breast adenocarcinoma cells MCF-7, and Chinese ... quickly put father in a spotWebHistone H3 phospho S10 Antibody Rabbit Polyclonal Item No. 600-401-I74 Size: 50 µg Applications: Dot Blot, IF, WB Reactivity: C. elegans, Human Host Species: Rabbit Have a … shipworks return labelWebAnti-Histone H3 (phospho S10) antibody [EPR17246] - ChIP Grade (ab177218) Research with confidence – consistent and reproducible results with every batch. Long-term and … shipworks pricing